Blues U21s will play Sheffield United away in the inaugural U21 Premier League Cup.
The tie will take place at the Blades' Bramall Lane Stadium on Monday 28 October, with a 1pm kick-off time.
Sheffield United have confirmed that supporters can attend the match free of charge.
This the first year that the U21 Premier League Cup has been held and it will be contested by 72 clubs.
The competition was created by the Premier League as an U21 equivalent to the FA Youth Cup, which is for U18 sides.