With the match being played just a day ahead of Blues' first team fixture against QPR, a youthful squad has made the trip up to South Yorkshire. It will be managed on the day by Academy coach Karl Hooper.
The game is being played at Wednesday’s Middlewood Road training facility and kicks-off at 1pm.
When the sides met in the corresponding U21 professional Development League Two fixture at Wast Hills in December, Blues ran out 3-1 winners.
The full Blues squad is: Preston, Trueman, Arthur, Sutherland, Kandi, McFarlane, Hales, O'Neill, McGee, Kelly, Deadfield, Truslove, Webb, Cooper, Blake, Cogley.