Blues face Solihull Moors at The Automated Technology Group Stadium (7.45pm), a game the manager is looking forward to.
The group that travelled to the camp in Marbella will be on duty once more, including trialist Dwight Tiendalli.
"It's always nice to get going with the games again and we know Solihull will be a good test," said Rowett.
"We will do what we did in Spain: we will look to give every player 45 minutes and do exactly the same on Saturday and then crack on with it.
"The players have worked extremely hard so far and again, yesterday, they had two demanding sessions.
"We will check on one or two - Ryan Shotton and Stephen Gleeson - who are a bit sore, but we anticipate everyone being available."
Tiendalli flew out to Spain with Blues. The defender, who can play left-back or right-back, is a free agent.
Said Rowett: "Dwight, as we know, is coming back from a knee problem he had last season. We've given him a couple of weeks to feel his way into it.
"There's no commitment from either side, it's just a case of having a look at him and him having a look at us."
Rowett fielded a different side in each half in Marbella against Hamilton Academical, who were sharing their training base. The game, arranged at the last minute when Blues were out there, ended 0-0.
Blues - who will be sporting the new red adidas away kit this evening - have become familiar opponents of Solihull.
Blues Under-21s played a pre-season friendly there last year and Marcus Bignot's side triumped 2-1 at St. Andrew's in the final of the Birmingham Senior Cup in May.
Solihull, winners of the Vanarama National North last season, drew 1-1 against Oxford United on Saturday in their opening friendly. Lewis Hayden equalised a goal by ex-Blues striker Wes Thomas.
Blues squad: Kuszczak, Legzdins, Trueman, Caddis, Grounds, Tiendalli, Spector, Morrison, Shotton, Robinson, Tesche, Davis, Gleeson, Adams, Kieftenbeld, Maghoma, Fabbrini, Solomon-Otabor, Arthur, Shinnie, Donaldson, Brock-Madsen, Vaughan.